HPLC stands for High Performance Liquid Chromatography system. This is an analytical chemistry technique that is used to separate, quantify and identify every component that is in a mixture. The technique depends on pumps to push some constrain liquid solvent which contains a mixture of the sample through a column containing a solid absorbent material.
In manufacturing, HPLC is mostly used in the process of production of biological and also pharmaceutical products. In medicine, it is used in detecting the level of vitamin D in blood serum. When it comes to research, HPLC is used for a couple of purposes. A good example is; separating components in biological samples, most of which are complex.
High Performance Liquid Chromatography is used in separating and purifying compounds according to their polarity. To understand polarity, we can use a simple example of oil, water and ethanol. Ethanol is polar. Thus it will mix properly with water. On the contrary, oil is not polar, so it does not mix well with water.
High Performance Liquid Chromatography system can be used in separating compounds in environmental samples, medical chemistry, microbiology and also environmental samples. The compounds are injected into the HPLC column. The mobile phase, which is practically a mixture of water and an organic solvent is then introduced. The organic solvent used is mostly methanol or acetonitrile. This is called the mobile phase since the mixture moves all through the HPLC column and still flushes out the compounds in the column at the same time. The process of flushing out is also known as eluting.
Related: Difference between C8 and C18 Columns Used in HPLC System
Compounds are frequently eluted alongside a concentration gradient. In simpler terms, the amount of water which is in the mobile phase decreases after some time, as the non-polar solvent percentage increases synchronously. The components in the mobile phase will then become more non-polar. The compounds that are contained in the sample used will stick to the carbon chain in the column. The compounds that are most non-polar will stick strongest while the most polar compounds will weakly.
Related: 10 Tips for HPLC Analysis In Pharmaceuticals
In manufacturing, HPLC is mostly used in the process of production of biological and also pharmaceutical products. In medicine, it is used in detecting the level of vitamin D in blood serum. When it comes to research, HPLC is used for a couple of purposes. A good example is; separating components in biological samples, most of which are complex.High Performance Liquid Chromatography is used in separating and purifying compounds according to their polarity. To understand polarity, we can use a simple example of oil, water and ethanol. Ethanol is polar. Thus it will mix properly with water. On the contrary, oil is not polar, so it does not mix well with water.
High Performance Liquid Chromatography system can be used in separating compounds in environmental samples, medical chemistry, microbiology and also environmental samples. The compounds are injected into the HPLC column. The mobile phase, which is practically a mixture of water and an organic solvent is then introduced. The organic solvent used is mostly methanol or acetonitrile. This is called the mobile phase since the mixture moves all through the HPLC column and still flushes out the compounds in the column at the same time. The process of flushing out is also known as eluting.
Related: Difference between C8 and C18 Columns Used in HPLC System
Compounds are frequently eluted alongside a concentration gradient. In simpler terms, the amount of water which is in the mobile phase decreases after some time, as the non-polar solvent percentage increases synchronously. The components in the mobile phase will then become more non-polar. The compounds that are contained in the sample used will stick to the carbon chain in the column. The compounds that are most non-polar will stick strongest while the most polar compounds will weakly.
Related: 10 Tips for HPLC Analysis In Pharmaceuticals
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