1.0 OBJECTIVE
To Verify system suitability values manually v/s software.2.0 SCOPE
This procedure is applicable to verify system suitability software results Vs manually of the high-performance liquid chromatography.3.0 RESPONSIBILITY
3.1 Doing: Technical Assistant3.2 Checking: Executive/ Manager
4.0 ACCOUNTABILITY
Head of the Department5.0 PROCEDURE
5.1 Frequency: Once in 6 months.5.2 Operate the instrument as per respective standard operating procedure of HPLC.
5.3 Prepare the mobile phase consisting of 70% methanol. : 30 % of water.
5.4 Prepare a mixture of 0.1 % Benzene and 0.1% Toluene in methanol.
5.5 Set flow rate at 1.0 ml/min.
5.6 Allow the system to be saturated with mobile phase for at least 15 minutes before injecting the test sample.
5.7 Record the area and retention time of both Benzene and Toluene in methanol.
5.8 Calculate the system suitability parameters with the help of software such as resolution, tailing factor and theoretical plate.
5.9 Now calculate the tailing factor, resolution and theoretical plate manually by following formula:
Theoretical Plate : N = 16 (t/w)2
Where t = R.T of the component
w = width of the component
Resolution : 2(t2 – t1)/ w1 + w2
Where t1 = R.T of Ist components
Where t1 = R.T of Ist components
t2 = R.T of IInd components
w1 = Width of Ist components
w2 = Width of IInd component
Tailing : T = w/2f
Where w = width of peak
f = width of half peak at 5 % height
The performance of software of the instrument is satisfactory if the all the parameters of the system suitability test are within the acceptance criteria.
Related: Calculation of Relative Response Factor (RRF) in HPLC
Related: Calculation of Relative Response Factor (RRF) in HPLC
Verification of system suitability parameters
2 (t2 – t1)
= ----------------- ( +/- 10 %)
2. Tailing :
By manually = T = w/2f
As per software =______________
Remarks: Comply/Does not comply
6.0 ABBREVIATION
6.1 % = Percentage6.2 ml = millilitre
6.3 RT = Retention time
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