Test for Free Formaldehyde (Formaldehyde Content) : Pharmaceutical Guidelines
  •   Pharmaguideline Courses Enroll Now
  • Aug 16, 2011

    Test for Free Formaldehyde (Formaldehyde Content)

    Learn hoe to determine the Formaldehyde Content by color development method.

    Free Formaldehyde

    Use Method A unless otherwise specified.

    Method A

    To 1.0 ml of a ten-fold dilution of the preparation under examination in a test-tube add 4.0 ml of water and 5.0 ml of acetylacetone reagent. Warm in a water-bath at 40° and allow to stand for 40 minutes. The solution is not more intensely colored than a reference solution prepared at the same time and in the same manner using 1.0 ml of a solution containing 0.002 percent w/v of formaldehyde, CH2O, in place of the dilution of the preparation. The comparison should be made examining the tubes down their vertical axes.

    Method B

    To 1.0 ml of ten-fold dilution of the preparation under examination in a test-tube add 2.0 ml of water, 1.0 ml of a 1 percent w/v solution of phenylhydrazine hydrochloride, 0.5 ml of a 5 percent w/v solution of potassium ferricyanide and 1.0 ml of hydrochloric acid and allow to stand for 15 minutes. The solution is not more intensely colored than a reference solution prepared at the same time and in the same manner using 1.0 ml of a solution containing 0.002 percent w/v of formaldehyde, CH2O, in place of the dilution of the preparation.
    The comparison should be made examining the tubes down their vertical axes.

    2 comments: Post Yours! Read Comment Policy ▼

    1. Hi Ankur,
      Could you please suggest if I have to conduct the test on a textile substrate then how do I perform it ?
      Awaiting for your reply.
      Regards,
      Pushkar

      ReplyDelete
    2. @Pushkar You can use above method to test formaldehyde.

      ReplyDelete

    Please don't spam. Comments having links would not be published.


    Popular Categories

    QA SOPs QC SOPs Micro SOPs HVAC Production SOPs Stores SOPs Checklists Maintenance SOPs HPLC Sterile GLP Validation Protocols Water System GDP Regulatory Maintenance Calibration Warning Letters Education B.Pharmacy
    Online Courses Salary Calculator
    Follow on Whatsapp


    Follow Pharmaguideline



    DOCUMENTS

    PHARMACEUTICAL DOCUMENTS




    Editable Pharmaceutical Documents in MS-Word Format. Ready to use SOPs, Protocols, Master Plans, Manuals and more...

    View


    adsbypg


    GET APP FOR NEWS UPDATES


    Android App