Reagent required
3N Hydrochloric acid0.05M Disodium Edetate
Sodium hydroxide solution
Hydroxy naphthol blue
Procedure
Dissolve about 350 mg of Calcium Citrate, previously dried at 150°C to constant weight and accurately weighed, in a mixture of 10 ml of water and 2 ml of 3N hydrochloric acid, and dilute with water to about 100 ml. While stirring, add about 30 ml of 0.05 M Edetate Disodium from a 50 ml burette. Add 15 ml of 1N sodium hydroxide and 300 mg of hydroxy naphthol blue as an indicator, and continue the titration to a blue endpoint. Each ml of 0.05 M Edetate Disodium is equivalent to 8.307 mg of Ca3(C6H5O7)2.Calculation
V x M x F 100
% Assay = -------------------- x----------------
0.05 x W (100-LOD)
Where,
V = Blank reading – Sample reading
M = Molarity of 0.05M Disodium Edetate
F = Factor
W = Weight of sample
% Assay = -------------------- x----------------
0.05 x W (100-LOD)
Where,
V = Blank reading – Sample reading
M = Molarity of 0.05M Disodium Edetate
F = Factor
W = Weight of sample
What is the effect of adding sodium hydroxide after 30ml EDTA
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