Determination of Peroxide Value : Pharmaguideline

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Determination of Peroxide Value

Learn how to determine the Peroxide Value in Pharmaceutical substance.
The peroxide value is the number of milliequivalents of active oxygen that expresses the amount of peroxide contained in 1000 g of the substance.

Method

Unless otherwise specified in the individual monograph, weigh accurately about 5 g of the substance under examination, transfer to a 250-ml glass-stoppered conical flask, add 30 ml of a mixture of 3 volumes of glacial acetic acid and 2 volumes of chloroform, swirl until dissolved and add 0.5 ml of saturated potassium iodide solution. Allow to stand for exactly 1 minute, with occasional shaking, add 30 ml of water and titrate gradually, with continuous and vigorous shaking, with 0.01 M sodium thiosulphate until the yellow color almost disappears. Add 0.5 ml of starch solution and continue the titration, shaking vigorously until the blue color just disappears. Carry out a blank titration omitting the substance under examination (b ml). The volume of 0.01 M sodium thiosulphate in the blank determination must not exceed 0.1 ml.
Calculate the peroxide value from the expression
Peroxide value = 10 (a - b)/w
Where, w = weight of the substance in g.





Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of pharmaguideline.com, a widely-read pharmaceutical blog since 2008. Sign-up for the free email updates for your daily dose of pharmaceutical tips.
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