Preparation and Standardization of 0.001 M Sodium Dodecyl Sulphate

Sodium Dodecyl Sulphate Solution Preparation

• Dissolve 0.2884 g of sodium dodecyl sulfate, calculated with reference to the substance dried at 105° for 2 hours, in sufficient water to produce 1000 ml. 
• Standardize the solution in the following manner.

Sodium Dodecyl Sulphate Solution Standardization

• To 50.0 ml add 15 ml of chloroform, 10 ml of 1 M sulphuric acid and 1 ml of dimethyl yellow-oracet blue solution.
• Titrate with 0.004 M benzethonium chloride, shaking vigorously and allowing the layers to separate after each addition until the chloroform layer acquires a permanent clear green color.
• 1 ml of 0.004 M benzethonium chloride is equivalent to 0.001154 g of C=H25NaO4S.

Also see: Determination of Shelf Life of Solutions in Laboratory