SOP for Start up after Shut Down in Microbiology : Pharmaguideline

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SOP for Start up after Shut Down in Microbiology

Standard operating procedure to start up the working after any shout down in Microbiology laboratory.

1.0 PURPOSE

To lay down the procedure for start-up procedure after shut down.

2.0 SCOPE

This procedure applies to microbiology lab for carrying out the procedure for shutdown and start-up of the microbiology lab.

3.0 RESPONSIBILITY

Microbiologist

4.0 ACCOUNTABILITY

Head of Department

5.0 PROCEDURE

5.1 Definitions

5.1.1 Major Shut Down: The activities which have a major impact on the quality of the microbiology lab environment in terms of viable and non viable particulate counts and includes activities such as
5.1.1.1 Civil works in the area.
5.1.1.2 Major Maintenance activity
5.1.1.3 HVAC shutdown / Power failure for more than 12 hours
5.1.2 Minor Shut Down: The activities which have a low impact on the quality of the microbiology lab environment in terms of viable and non viable particulate counts and includes activities such as
5.1.2.1 HVAC shutdown for less than 12 hours.
5.1.2.2 Preventive maintenance activity.
5.1.2.3 Minor modifications in the area.
5.1.2.4 Power failure for less than 12 hours.
5.1.3 Interruptions: The activities which have very low or no impact on the quality of the microbiology lab environment in terms of viable and non-viable particulate counts and includes activities such as
5.1.3.1 HVAC shutdown for less than 30 minutes.
5.1.3.2 Minor maintenance activity.
5.1.3.3 Power failure for less than 30 minutes.
5.1.3.4 Validation activity.

5.2 Procedure

5.2.1 Procedure for restart after major shut down

5.2.1.1 For restart after major shut down thorough cleaning of floors, walls, ceiling, doors and window panels by mopping with purified water and detergents.
5.2.1.2 Instruct Engineering personnel to Start Air Handling units.
5.2.1.3 Allow the area to be purged for at least 1 hour.
5.2.1.4 Re-clean and sanitize the area as per SOP ensuring that the floors, walls and ceiling are cleaned and sanitized.
5.2.1.5 Clean all the equipment present in the lab thoroughly externally and internally as per their respective SOP’s, if required.
5.2.1.6 Perform microbial environmental monitoring SOP.
5.2.1.7 Perform Non-viable particle counts in the area. Particle counting should be carried out in all rooms.
5.2.1.8 Testing operations should be performed only after achieving the environmental conditions suitable for non-viable and viable particle counts.
5.2.1.9 The final approval to start the area should be given by Quality Assurance after reviewing all the data and ensuring compliance with the requirements.

5.2.2 Procedure for restart after minor Shut Down

5.2.2.1 Before taking the lab under operation after minor shut down following operations should be carried out.
5.2.2.2 Ensure that the Air Handling units are in operation.
5.2.2.3 Allow the area to be purged for at least 1 hr.
5.2.2.4 Clean and sanitizes the area as per SOP ensuring that all the floors, walls and ceiling are cleaned and sanitized.
5.2.2.5 Clean all the equipment present in the lab thoroughly externally and internally as per their respective SOP’s, if required.
5.2.2.6 Perform microbial environmental monitoring SOP
5.2.2.7 Perform Non-viable particle counts in the area. Particle counting should be carried out in all rooms.
5.2.2.8 Testing operations should be performed after achieving the environmental conditions suitable for non-viable particle counts.
5.2.2.9 The final approval to start the area should be given by Quality Assurance after reviewing all the data and ensuring compliance with the requirements.

5.2.3 Procedure for restart after Interruptions.

5.2.3.1 Before taking the lab operation after interruptions, following activity should be carried out.
5.2.3.2 Ensure that the Air Handling units are in operation.
5.2.3.3 Clean and sanitizes the area as per SOP ensuring that the floors and walls are cleaned and sanitized.
5.2.3.4 Perform microbial environmental monitoring as per SOP
5.2.3.5 Testing operations can be initiated after Quality Assurance checks for proper cleaning.

6.0 ABBREVIATIONS

6.1 SOP - Standard Operating Procedure
6.2 HVAC - Heating Ventilating Air Conditioning
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