SOP for Proper Disposal of Contaminated Material Containing Microbial Growth

1.0 OBJECTIVE

To lay down a procedure for proper disposal of contaminated material to ensure all media containing microbial growth are disposed of properly.

2.0 SCOPE

This procedure is applicable for Disposal of contaminated material in Microbiology Laboratory of Quality Control department.

3.0 RESPONSIBILITY

Microbiologist

4.0 ACCOUNTABILITY

Head – QA & QC

5.0 PROCEDURE

5.1 Disposal procedure for contaminated Glassware

5.1.1 Wear latex gloves and disposable facemask.
5.1.2 Collect all microbiologically contaminated materials including Petri-plates, tubes, lyophilized cultures ampoules, culture slants/ stabs, flasks.
5.1.3 Put all the material into Himedia dispo bags and put the dispo bags into the vertical portable autoclave.
5.1.4 Run the vertical portable autoclave according to SOP for 30 minutes.
5.1.5 After completion of Autoclaving, the liquid medium should be drained while hot, with running water and then wash the reusable glassware as per procedure for washing of glassware according to SOP.

5.2 Disposal procedure for contaminated plasticware

5.2.1 Take all the disposable material into an autoclavable plastic bag and put all the disposable contaminated material that and run the vertical portable autoclave according to SOP and send all the material to disposal storage area.

5.3 Disposal of media filled ampoules/ vials

5.3.1 Autoclave the contaminated vials in the vertical portable autoclave.
5.3.2 The non contaminated vials should be drained in the effluent treatment plant and empty vials should be crushed in vial crusher.

5.4 Disposal of contaminated mops and latex gloves

5.4.1 The contaminated mops/tissue papers and latex gloves are used over the disinfected cultures on surfaces during any spillage and shall be put in poly bags before putting in discard bins and sent all the material to disposal storage area.

5.5 Disposal of Biological indicators

5.5.1 The biological indicators run as positive controls or indicators showing growth after running in sterilization cycles (if any) during validation studies shall be discarded after autoclaving along with other discard.

5.6 Sterility canister

5.6.1 The media of sterility canisters not showing any growth shall be drained into the sink after adding 5-10 ml of 2.5 % disinfectant solution as such per 100 ml of media to be discarded.
Sterility canisters showing growth shall be placed in an autoclavable plastic bag and put the bag in the vertical portable autoclave.
5.6.3 Run the vertical portable autoclave according to SOP for 30 minutes
5.6.4 Send the empty canisters, vials, needle and other waste articles to disposal storage area.

6.0 ABBREVIATIONS

SOP: Standard Operating Procedure
QA: Quality Assurance
QC: Quality Control
NA: Not Applicable
SS: Stainless steel

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