SOP for Calibration of Atomic Absorption Spectrophotometer (AAS)

1.0 Objective

To check the performance of the equipment for reliable and accurate results.

2.0 Scope

This procedure is applicable to check the performance of the atomic absorption spectrophotometer installed in the quality control department.

3.0 Responsibility

3.1 Doing: Technical Assistant
3.2 Checking: Executive /Manager

4.0 Accountability

Head of the Department

5.0 Procedure

Frequency: Once in a month
5.1 Operate the instrument as per respective standard operating procedure.
5.2 Use Cu standard for radiations.
5.3 Prepare 0.5 mcg/ml, 1.0 mcg/ml, 1.5 mcg/ml, 2.0 mcg/ml and 2.5 mcg/ml in water.
5.4 Aspirate the above solution and measure the absorbance.
5.5 Calculate the correlation coefficient by the instrument/computer.
5.6 Correlation coefficient should not less than 0.9900 %.
5.7 The performance of the instrument is satisfactory if the obtained correlation coefficient is within the given limit otherwise follow the S.O.P.

6.0 ABBREVIATIONS

6.1 % = Percentage
6.2 mcg= microgram
6.3 ml= millilitre
6.4 cu= copper

ANNEXURE-I

Q. C. Department

PERFORMANCE CHECK REPORT OF ATOMIC ABSORPTION

PERFORMANCE CHECK  DATE	DATE OF LAST PERFORMANCE CHECK DONE	NEXT DUE FOR PERFORMANCE CHECKE

INSTRUMENT DETAILS
INSTRUMENT NAME	INSTRUMENT MAKE	INSTRUMENT IDENTIFICATION NO.

OBSERVATION

Test % w/v	Absorption			Mean
	1	2	3
0.5 mcg/ml
1.0 mcg/ml
1.5 mcg/ml
2.0 mcg/ml
2.5 mcg/ml

 Correlation  coefficient : _________ (Limit : Not less than 0.9900 %)

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