Preparation and standardisation of 0.001 M Sodium Dodecyl Sulphate using Benzethonium Chloride and Dimethyl Yellow-Oracet Blue solution as indicator.
Sodium Dodecyl Sulphate Solution Preparation
- Dissolve 0.2884 g of sodium dodecyl sulfate, calculated with reference to the substance dried at 105° for 2 hours, in sufficient water to produce 1000 ml.
- Standardize the solution in the following manner.
Sodium Dodecyl Sulphate Solution Standardization
- To 50.0 ml add 15 ml of chloroform, 10 ml of 1 M sulphuric acid and 1 ml of dimethyl yellow-oracet blue solution.
- Titrate with 0.004 M benzethonium chloride, shaking vigorously and allowing the layers to separate after each addition until the chloroform layer acquires a permanent clear green color.
- 1 ml of 0.004 M benzethonium chloride is equivalent to 0.001154 g of C=H25NaO4S.
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Determination of Shelf Life of Solutions in Laboratory
Dr. Ankur Choudhary is a prominent Pharmaceutical Quality Assurance expert, consultant and the founder of Pharmaguideline. With over 22 years of hands-on experience in cGMP-compliant manufacturing environments, he specializes in establishing validation protocols, sterile area controls and data integrity systems. Ankur routinely interprets international regulatory frameworks (including FDA, EMA and ICH guidelines) to help global pharmaceutical professionals ensure strict regulatory compliance and operational excellence. Connect with Ankur on LinkedIn. Need Help: Ask Question
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